Dianne I. Greenfield

Associate Professor
Biological Oceanography

Science Building – Room E206
Phone: 212-413-3154
Email: DGreenfield@qc.cuny.edu or Dianne.Greenfield@asrc.cuny.edu
ASRC Weblink: http://environment.asrc.cuny.edu/people/dianne-greenfield/


Research Interests:

Phytoplankton, microscopic organisms that form the base of aquatic food webs, generate approximately half of the world’s oxygen through photosynthesis and exert considerable influence over nutrient and carbon cycling.  I study the complex feedbacks between global change stressors (such as urbanization, nutrients, and climate) and coastal phytoplankton ecology, physiology, and biogeochemistry. This includes but is not limited to the causes and consequences of ‘harmful algal blooms’ (HABs), events resulting in negative ecological, health, and/or economic impacts.  To achieve this goal, I develop and apply molecular tools to study plankton populations in situ and combine these advances with field and laboratory studies.  Since phytoplankton dynamics are central to ecosystem productivity, I also try to understand their linkages with trophic structure and function.

I am actively searching for talented, enthusiastic students (undergraduate and graduate) and researchers to join my group.  Please email me with questions and/or to schedule a phone call.


Courses Taught:

  • GEOL 370: Biogeochemistry
  • ENSCI 099: Practical Guide to Environmental Choices
  • GEOL 383: Coastal and Estuarine Science and Management


Teaching and Outreach Philosophy:

Oceanography is fun!  Interacting with students is the best part of my job, and I believe that students learn through multiple information sources and by active participation.  This includes discussions of primary literature, lectures, writing assignments, and hands-on field and laboratory experience.  I encourage students to explore scientific topics that they find interesting and to develop research questions and/or hypotheses around those subjects.   I also believe in substantial outreach and help students effectively communicate their findings through conferences, publications, workshops, and electronically.


Publications, Last 10 Years (visit my Google Citations for a complete list):

Lead authorship by students denoted as (*) my lab or ($) a colleague’s lab.


*Sitta, K., Callahan, T., Doll, C., Mortensen, R., Reed M., and D.I. Greenfield (2018) The influences of nitrogen form and zooplankton grazing on phytoplankton assemblages in two coastal southeastern systems. Limnology and Oceanography. 63: 2523-2544. DOI: 10.1002/lno.10957.

$Main, C., Greenfield, D.I., Doll, C., Wang, Y., Mortensen, R., Whereat, E.B., Pettay, D.T., and Coyne, K.C (2018) Critical comparison of molecular methods for detection and enumeration of the harmful algal species, Heterosigma akashiwo, in environmental water samples. Journal of Applied Phycology. 30: 2425-2434. DOI: 10.1007/s10811-018-1444-z.

$Van Meerssche, E., Greenfield, D.I. and J.L. Pinckney (2018) Coastal eutrophication and freshening: Impacts on Pseudo-nitzschia abundance and domoic acid allelopathy. Estuarine, Coastal, and Shelf Science. 209: 70-79. DOI: 10.1016/j.ecss.2018.05.013.

Pinckney, J.L., Tomas, C., Greenfield, D.I., Reale-Munroe, K., Castillo, B., Hillis-Starr, Z., Van Meerssche, E. and M. Zimberlin (2018) Seasonal changes in phytoplankton community structure in a bioluminescent lagoon, St. Croix, US Virgin Islands. Aquatic Microbial Ecology. 81: 109-124. DOI: 10.3354/ame01865.

$Ellis, K.K., Callahan, T., Greenfield, D.I., Sanger, D.M., Robinson, J. and M. Jones (2017) Measuring and modeling flow rates in tidal creeks: A case study from the central coast of South Carolina. Journal of South Carolina Water Resources. 4 (1) 21-39.

Greenfield, D.I., Moore, J., Stewart, J.R., Hilborn, E.D., George, B.J., Li, Q., Dickerson, J, Keppler, C.K. and P.A. Sandifer (2017) Temporal and environmental factors driving Vibrio vulnificus and V. parahaemolyticus populations and their associations with harmful algal blooms in South Carolina detention ponds and receiving tidal creeks. GeoHealth. 1 (9) 306-317. DOI: 10.1002/2017/GH000094.

*Reed, M., Pinckney, J.L., Keppler, C.K., Brock, L.M., Hogan, S.B. and D.I. Greenfield. (2016) The influence of nitrogen and phosphorus on seasonal phytoplankton biomass and community composition in four South Carolina systems. Estuarine, Coastal, and Shelf Science. 177: 71-82. DOI: 10.1016/j.ecss.2016.05.002.

Keppler, C.K., Bergquist, D.C., Brock, L.M., Felber, J. and D.I. Greenfield (2015) A spatial assessment of baseline nutrient and water quality values in the Ashepoo-Combahee-Edisto (ACE) Basin, South Carolina, USA. Marine Pollution Bulletin. 99: 332-337. DOI: 10.1016/j.marpolbul.2015.07.035.

Alber, M., Frischer, M., Greenfield, D.I., Hagy, J.D., Sheldon, J.E., Smith, E., Van Dolah, R.F. and C.B. Woodson (2015) GASCET: An approach to develop numeric nutrient criteria for Georgia and South Carolina estuaries. A task force report to the EPA, GA EPD, and SC DHEC. 59 pp.

*Reed, M., DiTullio, J., Kacenas, S. and D.I. Greenfield (2015) Effects of nitrogen and dissolved organic carbon on microplankton abundances in four coastal South Carolina systems. Aquatic Microbial Ecology. 76: 1-14. DOI: 10.3354/ame01764.

*Mortensen, R., Arnott, S., Jones, W.J. and D.I. Greenfield (2015) Development of a sandwich hybridization assay for the identification and quantification of red drum (Sciaenops ocellatus) eggs: a novel tool for fishery research and management. Canadian Journal of Fisheries and Aquatic Sciences. 72: 915-925. DOI: 10.1139/cjfas-2014-0526.

Greenfield, D.I., Keppler, C.K., Hilborn, E., Moore, J. and P. Sandifer (2014) Linking phytoplankton community composition with incidences of Vibrio in stormwater detentionponds. Proceedings of the 2014 South Carolina Water Resources Conference, Columbia, SC. October.

DeVoe, R.M., Callahan, T., Greenfield, D.I., Hitchcock, D., Peterson, R., Sanger, D., Turner, A., Wienstein, J. and E. Smith (2014) Stormwater management ponds in South Carolina: Formulating an integrated research and outreach collaborative to enhance pond ecology, Functionality, efficiency, effectiveness, and management. Proceedings of the 2014 South Carolina Water Resources Conference, Columbia, SC. October.

Greenfield, D.I., Duquette, A., Goodson, A., Keppler, C., Williams, S.H., Brock, L.M., Stackley, K.D., White, D. and S.B. Wilde (2014) The effects of three chemical algaecides on cell numbers and toxin content of the cyanobacteria Microcystis aeruginosa and Anabaenopsis sp. Environmental Management. 54: 1110-1120. DOI: 10.1007/s00267-014-0339-2.

$Main, C., Doll, D., Bianco, C., Greenfield, D.I. and K.J. Coyne (2014) The effects of growth phase, diel cycle and macronutrient stress on the quantification of Heterosigma akashiwo using qPCR and SHA. Harmful Algae. 37: 92-99. DOI: 10.1016/j.hal.2014.05.014.

*Doll, C., Main, C., Bianco, C., Coyne, K. and D.I. Greenfield (2014) Comparison of sandwich hybridization assay and quantitative PCR for the quantification of live and preserved cultures of Heterosigma akashiwo (Raphidophyceae). Limnology and Oceanography: Methods. 12: 232-245. DOI: 10.4319/lom.2014.12.232.

Key, P.B., Chung, K.W., Cooksey, C., DeLorenzo, M.E., Fulton, M.H., Greenfield, D.I., Greig, T., Hyland, J., Pennington, P.L., Petersen, E. and E.F. Wirth (2014) Assessment of crude oil and a dispersant in a simulated Spartina alterniflora salt marsh ecosystem. NOAA Technical Memorandum NOS NCCOS 186. 89 pp.

Pinckney, J.L., Greenfield, D.I., Benitez-Nelson, C., Long, R., Zimberlin, M., Lane, C., Thomas, C., Castillo, B, Reale-Munroe K., Taylor, M., Goldstein, D. and Z. Hillis-Starr (2014) Ecological characterization of bioluminescence in Mangrove Lagoon, Salt River Bay, St. Croix, USVI. NPS. 73 p.

Greenfield, D.I., Keppler, C., Brock, L.M., Reed, M., Kacenas, S., Hogan, S. and R. Van Dolah (2012) Assessing biological responses to nitrogen and phosphorus levels across the South Carolina coastal zone. Proceedings of the 2012 South Carolina Water Resources Conference, Columbia, SC. October.

Sanger, D.M., Smith, E.M., Voulgaris, G., Koepfler, E.T., Libes, S.M., Riekerk, G.H.M., Bergquist, D.C., Greenfield, D.I., Wren, P.A., McCoy, C.A., Viso, R.F.,  Peterson, R.N. and J.D. Whitaker (2012) Constrained enrichment contributes to hypoxia formation in Long Bay, South Carolina, an open water urbanized coastline. Marine Ecology Progress Series. 461: 15-30. DOI: 10.3354/meps09796.

$Siegel, A., Cotti-Rausch, B, Greenfield, D.I. and J. Pinckney (2011) Nutrient controls of     planktonic cyanobacteria abundance in coastal stormwater detention ponds. Marine Ecology Progress Series. 434: 15-27. DOI: 10.3354/meps09195.

Ryan, J., Greenfield, D.I., Marin, R. III, Preston, C., Birch, J., Doucette, G.J. and C.A. Scholin (2011) Harmful phytoplankton ecology studies using a molecular/environmental observing network. Limnology and Oceanography. 56: 1255-1272. DOI: 4319/lo.2011.56.4.1255.

Doucette, G.J., Mikulski, C.M., Jones, K.L, King, K.L., Greenfield, D.I., Marin, R. III, Jensen, S., Roman, B., Elliott, C.T. and C.A. Scholin (2009) Remote, subsurface detection of the algal toxin domoic acid onboard the Environmental Sample Processor: assay development and field trials. Harmful Algae. 8: 880-888. DOI: 10.1016/j.hal.2009.04.006.

Scholin, C.A., Doucette, G.J., Jensen, S., Roman, B., Pargett, D., Marin, R. III., Preston, C., Jones, W., Feldman, J., Everlove, C., Harris, A., Alvarado, N., Massion, E., Birch, J., Greenfield, D.I., Wheeler, K., Vrijenhoek, R., Mikulski, C. and K. Jones (2009) Remote detection of marine microbes, small invertebrates, harmful algae and biotoxins using the Environmental Sample Processor (ESP). NOPP Special Issue: Ocean Observing Platforms and Biosensors. Oceanography. 22 (2) 158-167. DOI: 10.5670/oceanog.2009.46.

Bergquist, D.C., Van Dolah, R.F., Riekerk, G.H.M., Levinson, M.V., Crowe, S.E., Brock, L.,Greenfield, D.I., Chestnut, D.E., McDermott, W., Fulton, M.H., Wirth, E. and J. Harvey (2009) The condition of South Carolina’s estuarine and coastal habitats during 2005 – 2006: Technical report. Charleston, SC: South Carolina’s Marine Resource Division. Technical Report No. 103. 74 p.

Greenfield, D.I., Marin, R. III., Doucette, G.J., Mikulski, C., Jensen, S., Roman, B., Alvarado, N., Feldman, J. and C.A. Scholin (2008) Field applications of the second-generation Environmental Sample Processor (ESP) for remote detection of harmful algae: 2006-2007. Limnology and Oceanography: Methods. 6: 667-679. DOI: 10.4319/lom.2008.6.667.


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